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Penghambatan Pelepasan Enzim -Hexoaminidase dari Sel Mast oleh Zeorin, Senyawa dari Aegla Marmelos Correa

2. On-line Journal of Universitas Islam Indonesia

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Title Penghambatan Pelepasan Enzim -Hexoaminidase dari Sel Mast oleh Zeorin, Senyawa dari Aegla Marmelos Correa
Creator Agung Endro Nugroho
Sugeng Riyanto
Mohd. Aspollah Hj.Sukari
Kazutaka Maeyama
Description Zeorin or 6,22-Dihydroxyhopane is a compound isolated from Aegle marmelos Correa collected in Yogyakarta Indonesia. The molecular structure was confirmed in Universiti Putra Malaysia. This compound was obtained from petroleum ether extract of the leaves of Aegle marmelos Correa. In present study, we investigated the effects of zeorin on the -hexoaminidase enzyme release from mast cell culture. The experiment was performed by using rat basophilic leukemia (RBL-2H3) cell line, a tumor analog of mast cells. DNP24-BSA and thapsigargin were used as immunologic and non-immunologic inducers for -hexoaminidase enzyme release from mast cells, respectively. The release of -hexoaminidase enzyme was determined by using colorimetric methods with an enzyme substrate, p-nitrofenil-2-Acetamido-2-deoksi--D-gluko-piranosida, and a microplate reader at 405 nm. In this study, treatment of 20 ng/mL DNP24-BSA and 0.5 M thapsigargin could stimulate the release of -hexoaminidase enzyme from RBL-2H3 cells by 25.421.62 % and 33.163.72 %, respectively. Zeorin showed potent inhibitory effects on the -hexoaminidase enzyme release, when the release induced by DNP24-BSA. In contrast, zeorin show weak inhibitory effects, when the -hexoaminidase enzyme release from RBL-2H3 cells induced by a Ca2+ stimulant, thapsigargin.nbsp; The IC50 values of zeorinrsquo;s effects on DNP24-BSA and thapsigargin experiments were 33,71 M and gt;100 M, respectively. Based on the results, the inhibitory effect of zeorin on the -hexoaminidase enzyme release from RBL-2H3 cells involving mechanisms related to the interaction of IgE on the mast cell surface or intracellular signal transductions involved in mast cell degranulation.Keywords : Aegle marmelos Correa, zeorin, sel mast,nbsp; b-hexoaminidase enzyme
Publisher Jurnal Ilmiah Farmasi
Date 2009-05-26
Type Peer-reviewed Article
Format application/pdf
Source Jurnal Ilmiah Farmasi; Vol 4, No 2 (2007)
Language en